[PulseNet Africa] Fwd: 1 selected item: 20737835 - PubMed

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Subject: 1 selected item: 20737835 - PubMed
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*Sender's message:* this is proof of campylobacter work in Uganda
Sent on: Wed Jun 18 10:27:54 2014

1 selected item: 20737835


PubMed ResultsItem 1 of 1    (Display the citation in PubMed
<http://www.ncbi.nlm.nih.gov/pubmed/20737835>)

1. Tanzan J Health Res. 2010 Jan;12(1):100-3.Role of microscopic
examination of stool specimens in the diagnosis of campylobacter infection
from children with acute diarrhoea in Kampala, Uganda.Mshana SE
<http://www.ncbi.nlm.nih.gov/pubmed?term=Mshana%20SE%5BAuthor%5D&cauthor=true&cauthor_uid=20737835>
1, Joloba ML
<http://www.ncbi.nlm.nih.gov/pubmed?term=Joloba%20ML%5BAuthor%5D&cauthor=true&cauthor_uid=20737835>,
Kakooza A
<http://www.ncbi.nlm.nih.gov/pubmed?term=Kakooza%20A%5BAuthor%5D&cauthor=true&cauthor_uid=20737835>,
Kaddu-Mulindwa D
<http://www.ncbi.nlm.nih.gov/pubmed?term=Kaddu-Mulindwa%20D%5BAuthor%5D&cauthor=true&cauthor_uid=20737835>
.

Author information:
1Department of Medical Microbiology, Makerere University Medical School,
Kampala, Uganda. mshana72 at yahoo.com
Abstract

Campylobacter species are a frequent cause of enteritis and less often of
extraintestinal infections in humans. The diagnosis of campylobacter
infection depends mainly on culture which is difficult and expensive to be
done as routine in most clinical microbiology laboratories in the
developing countries. This study was conducted to determine the sensitivity
and specificity of Gram-stain of the stool in diagnosis of campylobacter
infection, using culture as the gold standard. A total of 226 stool
specimens were obtained from children with acute diarrhoea, attending
Mulago Hospital in Kampala, Uganda. Stool smears were made and conventional
Gram stain done using 0.3% carbol-fuschin as counter stain for 5 minutes.
Mucous part of the stool was cultured in Charcoal Ceferaperazone
Deoxycholate Agar and blood contained selective media. A total of 21 stool
samples (9.3%) were positive by culture and 17 (7.5%) by Gram stain.
Sensitivity and specificity of Gram stain in the diagnosis of campylobacter
infection was 76% and 99.5%, respectively with positive predictive value of
94.1%. A total of 127 (56.2%) had white blood cells (WBC) in stool and
there was strong association between WBC in stool and the presence of
campylobacter infection (P=0.001). Gram stain is a good alternative in
diagnosis of campylobacter infection in place where facilities for culture
are limited.
PMID: 20737835 [PubMed - indexed for MEDLINE]
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