From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 09:35:55 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 07:35:55 +0000
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
Message-ID: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>

FYI.

Dr. Anthony Smith
Principal Medical Scientist
Centre for Enteric Diseases (Bacteriology Division)
National Institute for Communicable Diseases
South Africa
E-mail: anthonys at nicd.ac.za<mailto:anthonys at nicd.ac.za>
Tel: +27-11-5550348 | Cell: +27-83-4612145
Website: www.nicd.ac.za<http://www.nicd.ac.za/>


From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
Sent: 14 March 2019 09:04 PM
To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
Subject: New PulseNet WGS protocols

Dear all:
PulseNet USA has released several new WGS protocols. The original PNL32 protocol has been replaced by individual protocols for the different components of the WGS workflow. A procedure for the use of Illumina?s DNA Flex has also been released. We have received great feedback from the states on the use of the DNA Flex Library Prep kit.
Attached, please find all the protocols listed below. Feel free to share these with your network participants. In the near future, these protocols will be available on the PulseNet website. Let us know if you have questions about these protocols.

?         PNL33 - DNA Extraction and Quality

?         PNL34 ? PN Nextera XT Library Prep

?         PNL35 ? DNA Flex Library Prep

?         PNL38 -  WGS on MiSeq
Best,
Jeny

Jeniffer Concepci?n-Acevedo, Ph.D.
PulseNet International Coordinator<http://www.pulsenetinternational.org/> ? EDLB Communications Liaison
Centers for Disease Control and Prevention ?Enteric Diseases Laboratory Branch
P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov<mailto:ypi5 at cdc.gov>
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From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 10:15:38 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 08:15:38 +0000 (UTC)
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.4904.1552637419.10460.pulsenetafrica@lists.wits.ac.za>

 Dear Dr Anthony
Very great information and thank you
Best RegardsFirehiwot.



    On Friday, March 15, 2019, 10:38:00 AM GMT+3, pulsenetafrica at lists.wits.ac.za <pulsenetafrica at lists.wits.ac.za> wrote:  
 
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FYI.
 
 ?
 
Dr. Anthony Smith
 
Principal Medical Scientist
 
Centre for Enteric Diseases (Bacteriology Division)
 
National Institute for Communicable Diseases
 
South Africa
 
E-mail: anthonys at nicd.ac.za
 
Tel: +27-11-5550348 | Cell: +27-83-4612145
 
Website: www.nicd.ac.za
 
 ?
 
 ?
 
From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
Sent: 14 March 2019 09:04 PM
To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
Subject: New PulseNet WGS protocols
 
 ?
 
Dear all:
 
PulseNet USA has released several new WGS protocols. The original PNL32 protocol has been replaced by individual protocols for the different components of the WGS workflow. A procedure for the use of Illumina?s DNA Flex has also been released. We have received great feedback from the states on the use of the DNA Flex Library Prep kit.
 
Attached, please find all the protocols listed below. Feel free to share these with your network participants. In the near future, these protocols will be available on the PulseNet website. Let us know if you have questions about these protocols.
 
?????????PNL33 - DNA Extraction and Quality
 
?????????PNL34 ? PN Nextera XT Library Prep
 
?????????PNL35 ? DNA Flex Library Prep
 
?????????PNL38 - ?WGS on MiSeq
 
Best,
 
Jeny
 
 ?
 
Jeniffer Concepci?n-Acevedo, Ph.D.
 
PulseNet International Coordinator? EDLB Communications Liaison 
 
Centers for Disease Control and Prevention ?Enteric Diseases Laboratory Branch
P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
 _______________________________________________
Pulsenetafrica mailing list
Pulsenetafrica at lists.wits.ac.za
http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorised signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary.
  
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From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 10:35:30 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 11:35:30 +0300
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.4905.1552638626.10460.pulsenetafrica@lists.wits.ac.za>

Dear Sir
We are very grateful
Yours faithfully
Najjuka

On Fri, Mar 15, 2019 at 10:37 AM <pulsenetafrica at lists.wits.ac.za> wrote:

> FYI.
>
>
>
> *Dr. Anthony Smith*
>
> Principal Medical Scientist
>
> Centre for Enteric Diseases (Bacteriology Division)
>
> National Institute for Communicable Diseases
>
> South Africa
>
> E-mail: anthonys at nicd.ac.za
>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
>
> Website: www.nicd.ac.za
>
>
>
>
>
> *From:* Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
>
> *Sent:* 14 March 2019 09:04 PM
> *To:* Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <
> ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>;
> Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <
> pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <
> rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>;
> Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <
> Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> *Cc:* Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>;
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
> (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> *Subject:* New PulseNet WGS protocols
>
>
>
> Dear all:
>
> PulseNet USA has released several new WGS protocols. The original PNL32
> protocol has been replaced by individual protocols for the different
> components of the WGS workflow. A procedure for the use of Illumina?s DNA
> Flex has also been released. We have received great feedback from the
> states on the use of the DNA Flex Library Prep kit.
>
> Attached, please find all the protocols listed below. Feel free to share
> these with your network participants. In the near future, these protocols
> will be available on the PulseNet website. Let us know if you have
> questions about these protocols.
>
> ?         PNL33 - DNA Extraction and Quality
>
> ?         PNL34 ? PN Nextera XT Library Prep
>
> ?         PNL35 ? DNA Flex Library Prep
>
> ?         PNL38 -  WGS on MiSeq
>
> Best,
>
> Jeny
>
>
>
> *Jeniffer Concepci**?**n-Acevedo, Ph.D.*
>
> PulseNet International Coordinator <http://www.pulsenetinternational.org/> ?
> EDLB Communications Liaison
>
> Centers for Disease Control and Prevention ?Enteric Diseases Laboratory
> Branch
> P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is confidential.
> If you have received this communication in error, please notify us
> immediately and destroy the original message. You may not copy or
> disseminate this communication without the permission of the University.
> Only authorised signatories are competent to enter into agreements on
> behalf of the University and recipients are thus advised that the content
> of this message may not be legally binding on the University and may
> contain the personal views and opinions of the author, which are not
> necessarily the views and opinions of The University of the Witwatersrand,
> Johannesburg. All agreements between the University and outsiders are
> subject to South African Law unless the University agrees in writing to the
> contrary.
>
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From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 12:58:53 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 14:58:53 +0400
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.4950.1552647229.10460.pulsenetafrica@lists.wits.ac.za>

Thank you.
Regards
Sunita


*Mrs Sunita J Santchurn, PhD*

Senior Lecturer (Food Science & Technology)

Department of Agricultural & Food Science
Faculty of Agriculture
University of Mauritius
R?duit 80837
Republic of Mauritius

Tel: +230 403 7656
Fax: +230 465 5743
email: sunitab at uom.ac.mu

website: www.uom.ac.mu

Membre du r?seau QualiREG en Oc?an Indien: http://www.qualireg.org


On Fri, 15 Mar 2019 at 11:37, <pulsenetafrica at lists.wits.ac.za> wrote:

> FYI.
>
>
>
> *Dr. Anthony Smith*
>
> Principal Medical Scientist
>
> Centre for Enteric Diseases (Bacteriology Division)
>
> National Institute for Communicable Diseases
>
> South Africa
>
> E-mail: anthonys at nicd.ac.za
>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
>
> Website: www.nicd.ac.za
>
>
>
>
>
> *From:* Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
>
> *Sent:* 14 March 2019 09:04 PM
> *To:* Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <
> ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>;
> Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <
> pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <
> rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>;
> Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <
> Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> *Cc:* Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>;
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
> (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> *Subject:* New PulseNet WGS protocols
>
>
>
> Dear all:
>
> PulseNet USA has released several new WGS protocols. The original PNL32
> protocol has been replaced by individual protocols for the different
> components of the WGS workflow. A procedure for the use of Illumina?s DNA
> Flex has also been released. We have received great feedback from the
> states on the use of the DNA Flex Library Prep kit.
>
> Attached, please find all the protocols listed below. Feel free to share
> these with your network participants. In the near future, these protocols
> will be available on the PulseNet website. Let us know if you have
> questions about these protocols.
>
> ?         PNL33 - DNA Extraction and Quality
>
> ?         PNL34 ? PN Nextera XT Library Prep
>
> ?         PNL35 ? DNA Flex Library Prep
>
> ?         PNL38 -  WGS on MiSeq
>
> Best,
>
> Jeny
>
>
>
> *Jeniffer Concepci**?**n-Acevedo, Ph.D.*
>
> PulseNet International Coordinator <http://www.pulsenetinternational.org/> ?
> EDLB Communications Liaison
>
> Centers for Disease Control and Prevention ?Enteric Diseases Laboratory
> Branch
> P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is confidential.
> If you have received this communication in error, please notify us
> immediately and destroy the original message. You may not copy or
> disseminate this communication without the permission of the University.
> Only authorised signatories are competent to enter into agreements on
> behalf of the University and recipients are thus advised that the content
> of this message may not be legally binding on the University and may
> contain the personal views and opinions of the author, which are not
> necessarily the views and opinions of The University of the Witwatersrand,
> Johannesburg. All agreements between the University and outsiders are
> subject to South African Law unless the University agrees in writing to the
> contrary.
>

-- 
 <http://www.uom.ac.mu/index.php/email-disclaimer>
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From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 13:44:58 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 11:44:58 +0000 (UTC)
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.4951.1552649979.10460.pulsenetafrica@lists.wits.ac.za>

 Thanks, AnthonyBestStella

    On Friday, March 15, 2019, 8:38:02 AM GMT+1, pulsenetafrica at lists.wits.ac.za <pulsenetafrica at lists.wits.ac.za> wrote:  
 
  
FYI.
 
 ?
 
Dr. Anthony Smith
 
Principal Medical Scientist
 
Centre for Enteric Diseases (Bacteriology Division)
 
National Institute for Communicable Diseases
 
South Africa
 
E-mail: anthonys at nicd.ac.za
 
Tel: +27-11-5550348 | Cell: +27-83-4612145
 
Website: www.nicd.ac.za
 
 ?
 
 ?
 
From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
Sent: 14 March 2019 09:04 PM
To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
Subject: New PulseNet WGS protocols
 
 ?
 
Dear all:
 
PulseNet USA has released several new WGS protocols. The original PNL32 protocol has been replaced by individual protocols for the different components of the WGS workflow. A procedure for the use of Illumina?s DNA Flex has also been released. We have received great feedback from the states on the use of the DNA Flex Library Prep kit.
 
Attached, please find all the protocols listed below. Feel free to share these with your network participants. In the near future, these protocols will be available on the PulseNet website. Let us know if you have questions about these protocols.
 
?????????PNL33 - DNA Extraction and Quality
 
?????????PNL34 ? PN Nextera XT Library Prep
 
?????????PNL35 ? DNA Flex Library Prep
 
?????????PNL38 - ?WGS on MiSeq
 
Best,
 
Jeny
 
 ?
 
Jeniffer Concepci?n-Acevedo, Ph.D.
 
PulseNet International Coordinator? EDLB Communications Liaison 
 
Centers for Disease Control and Prevention ?Enteric Diseases Laboratory Branch
P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
 _______________________________________________
Pulsenetafrica mailing list
Pulsenetafrica at lists.wits.ac.za
http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorised signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary.
  
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From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 14:11:50 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 14:11:50 +0200
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4951.1552649979.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
 <mailman.4951.1552649979.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.4955.1552651613.10460.pulsenetafrica@lists.wits.ac.za>

Thank you so much, Antony

Regards

Dr. Agness Nhidza-Manjoro (PhD)

Cell: +263-772 114053



On Fri, Mar 15, 2019 at 1:45 PM <pulsenetafrica at lists.wits.ac.za> wrote:

> Thanks, Anthony
> Best
> Stella
>
> On Friday, March 15, 2019, 8:38:02 AM GMT+1,
> pulsenetafrica at lists.wits.ac.za <pulsenetafrica at lists.wits.ac.za> wrote:
>
>
> FYI.
>
>
>
> *Dr. Anthony Smith*
>
> Principal Medical Scientist
>
> Centre for Enteric Diseases (Bacteriology Division)
>
> National Institute for Communicable Diseases
>
> South Africa
>
> E-mail: anthonys at nicd.ac.za
>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
>
> Website: www.nicd.ac.za
>
>
>
>
>
> *From:* Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
>
> *Sent:* 14 March 2019 09:04 PM
> *To:* Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <
> ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>;
> Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <
> pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <
> rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>;
> Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <
> Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> *Cc:* Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>;
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
> (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> *Subject:* New PulseNet WGS protocols
>
>
>
> Dear all:
>
> PulseNet USA has released several new WGS protocols. The original PNL32
> protocol has been replaced by individual protocols for the different
> components of the WGS workflow. A procedure for the use of Illumina?s DNA
> Flex has also been released. We have received great feedback from the
> states on the use of the DNA Flex Library Prep kit.
>
> Attached, please find all the protocols listed below. Feel free to share
> these with your network participants. In the near future, these protocols
> will be available on the PulseNet website. Let us know if you have
> questions about these protocols.
>
> ?         PNL33 - DNA Extraction and Quality
>
> ?         PNL34 ? PN Nextera XT Library Prep
>
> ?         PNL35 ? DNA Flex Library Prep
>
> ?         PNL38 -  WGS on MiSeq
>
> Best,
>
> Jeny
>
>
>
> *Jeniffer Concepci**?**n-Acevedo, Ph.D.*
>
> PulseNet International Coordinator <http://www.pulsenetinternational.org/> ?
> EDLB Communications Liaison
>
> Centers for Disease Control and Prevention ?Enteric Diseases Laboratory
> Branch
> P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is confidential.
> If you have received this communication in error, please notify us
> immediately and destroy the original message. You may not copy or
> disseminate this communication without the permission of the University.
> Only authorised signatories are competent to enter into agreements on
> behalf of the University and recipients are thus advised that the content
> of this message may not be legally binding on the University and may
> contain the personal views and opinions of the author, which are not
> necessarily the views and opinions of The University of the Witwatersrand,
> Johannesburg. All agreements between the University and outsiders are
> subject to South African Law unless the University agrees in writing to the
> contrary.
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is confidential.
> If you have received this communication in error, please notify us
> immediately and destroy the original message. You may not copy or
> disseminate this communication without the permission of the University.
> Only authorised signatories are competent to enter into agreements on
> behalf of the University and recipients are thus advised that the content
> of this message may not be legally binding on the University and may
> contain the personal views and opinions of the author, which are not
> necessarily the views and opinions of The University of the Witwatersrand,
> Johannesburg. All agreements between the University and outsiders are
> subject to South African Law unless the University agrees in writing to the
> contrary.
>
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From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 14:22:30 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 12:22:30 +0000
Subject: [PulseNet Africa] New PulseNet WGS protocols
Message-ID: <mailman.4962.1552652212.10460.pulsenetafrica@lists.wits.ac.za>


Thanks - well received


On March 15, 2019 09:38:01 am GMT+2, pulsenetafrica at lists.wits.ac.za wrote:

> FYI.
>
> Dr. Anthony Smith
> Principal Medical Scientist
> Centre for Enteric Diseases (Bacteriology Division)
> National Institute for Communicable Diseases
> South Africa
> E-mail: anthonys at nicd.ac.za<mailto:anthonys at nicd.ac.za>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
> Website: www.nicd.ac.za<http://www.nicd.ac.za/>
>
>
> From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
> Sent: 14 March 2019 09:04 PM
> To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen 
> <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) 
> <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. 
> Enrique (WDC) <pereze at paho.org>; Amina AlJardani 
> <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 
> 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin 
> <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle 
> <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; 
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, 
> Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> Subject: New PulseNet WGS protocols
>
> Dear all:
> PulseNet USA has released several new WGS protocols. The original 
> PNL32 protocol has been replaced by individual protocols for the 
> different components of the WGS workflow. A procedure for the use of 
> Illumina?s DNA Flex has also been released. We have received great 
> feedback from the states on the use of the DNA Flex Library Prep kit.
> Attached, please find all the protocols listed below. Feel free to 
> share these with your network participants. In the near future, these 
> protocols will be available on the PulseNet website. Let us know if 
> you have questions about these protocols.
>
> ?         PNL33 - DNA Extraction and Quality
>
> ?         PNL34 ? PN Nextera XT Library Prep
>
> ?         PNL35 ? DNA Flex Library Prep
>
> ?         PNL38 -  WGS on MiSeq
> Best,
> Jeny
>
> Jeniffer Concepci?n-Acevedo, Ph.D.
> PulseNet International 
> Coordinator<http://www.pulsenetinternational.org/> ? EDLB 
> Communications Liaison
> Centers for Disease Control and Prevention ?Enteric Diseases 
> Laboratory Branch
> P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov<mailto:ypi5 at cdc.gov>
>
>
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is 
> confidential. If you have received this communication in error, 
> please notify us immediately and destroy the original message. You 
> may not copy or disseminate this communication without the permission 
> of the University. Only authorised signatories are competent to enter 
> into agreements on behalf of the University and recipients are thus 
> advised that the content of this message may not be legally binding 
> on the University and may contain the personal views and opinions of 
> the author, which are not necessarily the views and opinions of The 
> University of the Witwatersrand, Johannesburg. All agreements between 
> the University and outsiders are subject to South African Law unless 
> the University agrees in writing to the contrary.


--
Andrew Tarupiwa
tarupiwaa at nmrl.org.zw



From pulsenetafrica at lists.wits.ac.za  Fri Mar 15 17:16:56 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Fri, 15 Mar 2019 15:16:56 +0000 (UTC)
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.5005.1552662684.10460.pulsenetafrica@lists.wits.ac.za>

Dear ANthony,thank you.Best

Dr Kalpy Julien COULIBALY, MD, MSc FS, PhD
M?decin Microbiologiste / Charg? de Recherche
Chef de D?partement Environnement et Sant? 
Institut Pasteur de C?te d'Ivoire
01 BP 490 Abidjan 01 

Centre M?dico-Social
Universit? F?lix Houphouet-Boigny
BP V 34 Abidjan
kalpyjuliencoulibaly at pasteur.ci
0022507915176 

    Le vendredi 15 mars 2019 ? 07:38:00 UTC, pulsenetafrica at lists.wits.ac.za <pulsenetafrica at lists.wits.ac.za> a ?crit :  
 
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FYI.
 
 ?
 
Dr. Anthony Smith
 
Principal Medical Scientist
 
Centre for Enteric Diseases (Bacteriology Division)
 
National Institute for Communicable Diseases
 
South Africa
 
E-mail: anthonys at nicd.ac.za
 
Tel: +27-11-5550348 | Cell: +27-83-4612145
 
Website: www.nicd.ac.za
 
 ?
 
 ?
 
From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
Sent: 14 March 2019 09:04 PM
To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
Subject: New PulseNet WGS protocols
 
 ?
 
Dear all:
 
PulseNet USA has released several new WGS protocols. The original PNL32 protocol has been replaced by individual protocols for the different components of the WGS workflow. A procedure for the use of Illumina?s DNA Flex has also been released. We have received great feedback from the states on the use of the DNA Flex Library Prep kit.
 
Attached, please find all the protocols listed below. Feel free to share these with your network participants. In the near future, these protocols will be available on the PulseNet website. Let us know if you have questions about these protocols.
 
?????????PNL33 - DNA Extraction and Quality
 
?????????PNL34 ? PN Nextera XT Library Prep
 
?????????PNL35 ? DNA Flex Library Prep
 
?????????PNL38 - ?WGS on MiSeq
 
Best,
 
Jeny
 
 ?
 
Jeniffer Concepci?n-Acevedo, Ph.D.
 
PulseNet International Coordinator? EDLB Communications Liaison 
 
Centers for Disease Control and Prevention ?Enteric Diseases Laboratory Branch
P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
 _______________________________________________
Pulsenetafrica mailing list
Pulsenetafrica at lists.wits.ac.za
http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorised signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary.
  
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From pulsenetafrica at lists.wits.ac.za  Sat Mar 16 15:34:09 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Sat, 16 Mar 2019 13:34:09 +0000 (UTC)
Subject: [PulseNet Africa] New PulseNet WGS protocols
References: <1211078285.5961708.1552743249194.ref@mail.yahoo.com>
Message-ID: <mailman.5030.1552743033.10460.pulsenetafrica@lists.wits.ac.za>

Dear Anthony,  Thank you for this communication. Stephen Ndibowa 
--------------------------------------------
On Fri, 3/15/19,  <pulsenetafrica at lists.wits.ac.za> wrote:

 Subject: Re: [PulseNet Africa] New PulseNet WGS protocols
 To: "pulsenetafrica at lists.wits.ac.za" <pulsenetafrica at lists.wits.ac.za>
 Date: Friday, March 15, 2019, 6:16 PM
 
 
 
 
 
 
 
 This message is eligible for Automatic Cleanup!
 (pulsenetafrica at lists.wits.ac.za) 
 Add cleanup
 rule
  | More info
 
 
 
     
 
 Dear
 ANthony,thank you.Best
 
 Dr Kalpy
 Julien COULIBALY, MD, MSc FS, PhD
 M?decin Microbiologiste / Charg? de Recherche
 Chef de D?partement Environnement et Sant? 
 Institut Pasteur de C?te
 d'Ivoire
 01 BP 490 Abidjan 01 
 
 Centre M?dico-Social
 Universit? F?lix
 Houphouet-Boigny
 BP V 34 Abidjan
 kalpyjuliencoulibaly at pasteur.ci
 0022507915176
         
 
 
         
         
             
                 
                 
                     Le vendredi 15 mars 2019 ? 07:38:00
 UTC, pulsenetafrica at lists.wits.ac.za
 <pulsenetafrica at lists.wits.ac.za> a ?crit :
                 
                 
 
                 
 
                 
 
  
  
 
 
 
 
 FYI. 
  ? 
 
 Dr.
 Anthony Smith
 
 
 Principal
 Medical Scientist 
 
 Centre
 for Enteric Diseases (Bacteriology Division) 
 
 National
 Institute for Communicable Diseases 
 
 South
 Africa 
 
 E-mail:
 
 anthonys at nicd.ac.za
 
 
 Tel:
 +27-11-5550348 | Cell: +27-83-4612145 
 
 Website:
 
 www.nicd.ac.za 
  ? 
  ? 
 
 
 
 From:
 Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED)
 <ypi5 at cdc.gov>
 
 
 Sent: 14 March 2019 09:04 PM
 
 To: Nadon, Celine (PHAC/ASPC)
 <celine.nadon at canada.ca>; Isabel Chinen
 <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof.
 (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith
 <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC)
 <pereze at paho.org>; Amina AlJardani
 <aksaljardani at gmail.com>;
  Rajesh Kumar <rajeshverma2005 at gmail.com>;
 'Josefina Campos' <jocampos05 at gmail.com>;
 Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle
 <Ivo.Van.Walle at ecdc.europa.eu>;
 Johanna.Takkinen at ecdc.europa.eu
 
 Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED)
 <evy7 at cdc.gov>; Gerner-Smidt, Peter
 (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
 (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
 
 Subject: New PulseNet WGS protocols 
 
 
  ? 
 Dear all: 
 PulseNet USA has released several new WGS
 protocols. The original PNL32 protocol has been replaced by
 individual protocols for the different components of the
  WGS workflow. A procedure for the use of Illumina?s DNA
 Flex has also been released. We have received great feedback
 from the states on the use of the DNA Flex Library Prep kit.
  
 Attached, please find all the protocols listed
 below. Feel free to share these with your network
 participants. In the near future, these protocols will be
 available
  on the PulseNet website. Let us know if you have questions
 about these protocols.
  
 
 ?????????
 PNL33 - DNA Extraction and Quality 
 
 ?????????
 PNL34 ? PN Nextera XT Library Prep
 
 
 ?????????
 PNL35 ? DNA Flex Library Prep 
 
 ?????????
 PNL38 - ?WGS on MiSeq 
 Best, 
 Jeny 
 
 ? 
 
 Jeniffer Concepci?n-Acevedo, Ph.D. 
 
 PulseNet International Coordinator
 ? EDLB
 Communications Liaison 
  
 
 Centers for
 Disease Control and Prevention ?Enteric Diseases
 Laboratory Branch
 
 P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov 
 
 
 
 _______________________________________________
 Pulsenetafrica mailing list
 Pulsenetafrica at lists.wits.ac.za
 http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
 This communication is intended for the addressee only. It is
 confidential. If you have received this communication in
 error, please notify us immediately and destroy the original
 message. You may not copy or disseminate this communication
 without the permission of the University. Only authorised
 signatories are competent to enter into agreements on behalf
 of the University and recipients are thus advised that the
 content of this message may not be legally binding on the
 University and may contain the personal views and opinions
 of the author, which are not necessarily the views and
 opinions of The University of the Witwatersrand,
 Johannesburg. All agreements between the University and
 outsiders are subject to South African Law unless the
 University agrees in writing to the contrary.
 
             
        
 _______________________________________________
 Pulsenetafrica mailing list
 Pulsenetafrica at lists.wits.ac.za
 http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
 This communication is intended for the addressee only. It is
 confidential. If you have received this communication in
 error, please notify us immediately and destroy the original
 message. You may not copy or disseminate this communication
 without the permission of the University. Only authorised
 signatories are competent to enter into agreements on behalf
 of the University and recipients are thus advised that the
 content of this message may not be legally binding on the
 University and may contain the personal views and opinions
 of the author, which are not necessarily the views and
 opinions of The University of the Witwatersrand,
 Johannesburg. All agreements between the University and
 outsiders are subject to South African Law unless the
 University agrees in writing to the contrary.
 
 -----Inline Attachment Follows-----
 
 


From pulsenetafrica at lists.wits.ac.za  Mon Mar 18 10:39:50 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Mon, 18 Mar 2019 10:39:50 +0200
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.5030.1552743033.10460.pulsenetafrica@lists.wits.ac.za>
References: <1211078285.5961708.1552743249194.ref@mail.yahoo.com>
 <mailman.5030.1552743033.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.5071.1552898021.10460.pulsenetafrica@lists.wits.ac.za>

Thank Dr Anthony for the information
Daniel Lawadi Banda
Senior Lecturer in Medical Microbiology
Department of Medical Laboratory Sciences
College of Medicine
University of Malawi



Mobile:   +265 99 729 5898 For calling
              +265 88 459 6936 For calling
              +27 622946654 For WhatsApp
Skype:daniel.banda3



On Sat, Mar 16, 2019 at 3:36 PM <pulsenetafrica at lists.wits.ac.za> wrote:

> Dear Anthony,  Thank you for this communication. Stephen Ndibowa
> --------------------------------------------
> On Fri, 3/15/19,  <pulsenetafrica at lists.wits.ac.za> wrote:
>
>  Subject: Re: [PulseNet Africa] New PulseNet WGS protocols
>  To: "pulsenetafrica at lists.wits.ac.za" <pulsenetafrica at lists.wits.ac.za>
>  Date: Friday, March 15, 2019, 6:16 PM
>
>
>
>
>
>
>
>  This message is eligible for Automatic Cleanup!
>  (pulsenetafrica at lists.wits.ac.za)
>  Add cleanup
>  rule
>   | More info
>
>
>
>
>
>  Dear
>  ANthony,thank you.Best
>
>  Dr Kalpy
>  Julien COULIBALY, MD, MSc FS, PhD
>  M?decin Microbiologiste / Charg? de Recherche
>  Chef de D?partement Environnement et Sant?
>  Institut Pasteur de C?te
>  d'Ivoire
>  01 BP 490 Abidjan 01
>
>  Centre M?dico-Social
>  Universit? F?lix
>  Houphouet-Boigny
>  BP V 34 Abidjan
>  kalpyjuliencoulibaly at pasteur.ci
>  0022507915176
>
>
>
>
>
>
>
>
>                      Le vendredi 15 mars 2019 ? 07:38:00
>  UTC, pulsenetafrica at lists.wits.ac.za
>  <pulsenetafrica at lists.wits.ac.za> a ?crit :
>
>
>
>
>
>
>
>
>
>
>
>
>
>  FYI.
>
>
>  Dr.
>  Anthony Smith
>
>
>  Principal
>  Medical Scientist
>
>  Centre
>  for Enteric Diseases (Bacteriology Division)
>
>  National
>  Institute for Communicable Diseases
>
>  South
>  Africa
>
>  E-mail:
>
>  anthonys at nicd.ac.za
>
>
>  Tel:
>  +27-11-5550348 | Cell: +27-83-4612145
>
>  Website:
>
>  www.nicd.ac.za
>
>
>
>
>
>  From:
>  Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED)
>  <ypi5 at cdc.gov>
>
>
>  Sent: 14 March 2019 09:04 PM
>
>  To: Nadon, Celine (PHAC/ASPC)
>  <celine.nadon at canada.ca>; Isabel Chinen
>  <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof.
>  (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith
>  <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC)
>  <pereze at paho.org>; Amina AlJardani
>  <aksaljardani at gmail.com>;
>   Rajesh Kumar <rajeshverma2005 at gmail.com>;
>  'Josefina Campos' <jocampos05 at gmail.com>;
>  Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle
>  <Ivo.Van.Walle at ecdc.europa.eu>;
>  Johanna.Takkinen at ecdc.europa.eu
>
>  Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED)
>  <evy7 at cdc.gov>; Gerner-Smidt, Peter
>  (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
>  (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
>
>  Subject: New PulseNet WGS protocols
>
>
>
>  Dear all:
>  PulseNet USA has released several new WGS
>  protocols. The original PNL32 protocol has been replaced by
>  individual protocols for the different components of the
>   WGS workflow. A procedure for the use of Illumina?s DNA
>  Flex has also been released. We have received great feedback
>  from the states on the use of the DNA Flex Library Prep kit.
>
>  Attached, please find all the protocols listed
>  below. Feel free to share these with your network
>  participants. In the near future, these protocols will be
>  available
>   on the PulseNet website. Let us know if you have questions
>  about these protocols.
>
>
>  ?
>  PNL33 - DNA Extraction and Quality
>
>  ?
>  PNL34 ? PN Nextera XT Library Prep
>
>
>  ?
>  PNL35 ? DNA Flex Library Prep
>
>  ?
>  PNL38 -  WGS on MiSeq
>  Best,
>  Jeny
>
>
>
>  Jeniffer Concepci?n-Acevedo, Ph.D.
>
>  PulseNet International Coordinator
>  ? EDLB
>  Communications Liaison
>
>
>  Centers for
>  Disease Control and Prevention ?Enteric Diseases
>  Laboratory Branch
>
>  P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
>
>
>
>  _______________________________________________
>  Pulsenetafrica mailing list
>  Pulsenetafrica at lists.wits.ac.za
>  http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
>  This communication is intended for the addressee only. It is
>  confidential. If you have received this communication in
>  error, please notify us immediately and destroy the original
>  message. You may not copy or disseminate this communication
>  without the permission of the University. Only authorised
>  signatories are competent to enter into agreements on behalf
>  of the University and recipients are thus advised that the
>  content of this message may not be legally binding on the
>  University and may contain the personal views and opinions
>  of the author, which are not necessarily the views and
>  opinions of The University of the Witwatersrand,
>  Johannesburg. All agreements between the University and
>  outsiders are subject to South African Law unless the
>  University agrees in writing to the contrary.
>
>
>
>  _______________________________________________
>  Pulsenetafrica mailing list
>  Pulsenetafrica at lists.wits.ac.za
>  http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
>  This communication is intended for the addressee only. It is
>  confidential. If you have received this communication in
>  error, please notify us immediately and destroy the original
>  message. You may not copy or disseminate this communication
>  without the permission of the University. Only authorised
>  signatories are competent to enter into agreements on behalf
>  of the University and recipients are thus advised that the
>  content of this message may not be legally binding on the
>  University and may contain the personal views and opinions
>  of the author, which are not necessarily the views and
>  opinions of The University of the Witwatersrand,
>  Johannesburg. All agreements between the University and
>  outsiders are subject to South African Law unless the
>  University agrees in writing to the contrary.
>
>  -----Inline Attachment Follows-----
>
>
>
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is confidential.
> If you have received this communication in error, please notify us
> immediately and destroy the original message. You may not copy or
> disseminate this communication without the permission of the University.
> Only authorised signatories are competent to enter into agreements on
> behalf of the University and recipients are thus advised that the content
> of this message may not be legally binding on the University and may
> contain the personal views and opinions of the author, which are not
> necessarily the views and opinions of The University of the Witwatersrand,
> Johannesburg. All agreements between the University and outsiders are
> subject to South African Law unless the University agrees in writing to the
> contrary.
>
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From pulsenetafrica at lists.wits.ac.za  Mon Mar 18 11:05:49 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Mon, 18 Mar 2019 10:05:49 +0100
Subject: [PulseNet Africa] New PulseNet WGS protocols
In-Reply-To: <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
References: <ab8d68cf9e06443db228e614ff4ef221@cdc.gov>
 <mailman.4898.1552635110.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.5082.1552899621.10460.pulsenetafrica@lists.wits.ac.za>

Receipt acknowledged with very many thanks.
Opeoluwa Shonekan
Opeolu A. Shonekan


On Fri, Mar 15, 2019 at 8:37 AM <pulsenetafrica at lists.wits.ac.za> wrote:

> FYI.
>
>
>
> *Dr. Anthony Smith*
>
> Principal Medical Scientist
>
> Centre for Enteric Diseases (Bacteriology Division)
>
> National Institute for Communicable Diseases
>
> South Africa
>
> E-mail: anthonys at nicd.ac.za
>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
>
> Website: www.nicd.ac.za
>
>
>
>
>
> *From:* Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
>
> *Sent:* 14 March 2019 09:04 PM
> *To:* Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <
> ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>;
> Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <
> pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <
> rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>;
> Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <
> Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> *Cc:* Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>;
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
> (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> *Subject:* New PulseNet WGS protocols
>
>
>
> Dear all:
>
> PulseNet USA has released several new WGS protocols. The original PNL32
> protocol has been replaced by individual protocols for the different
> components of the WGS workflow. A procedure for the use of Illumina?s DNA
> Flex has also been released. We have received great feedback from the
> states on the use of the DNA Flex Library Prep kit.
>
> Attached, please find all the protocols listed below. Feel free to share
> these with your network participants. In the near future, these protocols
> will be available on the PulseNet website. Let us know if you have
> questions about these protocols.
>
> ?         PNL33 - DNA Extraction and Quality
>
> ?         PNL34 ? PN Nextera XT Library Prep
>
> ?         PNL35 ? DNA Flex Library Prep
>
> ?         PNL38 -  WGS on MiSeq
>
> Best,
>
> Jeny
>
>
>
> *Jeniffer Concepci**?**n-Acevedo, Ph.D.*
>
> PulseNet International Coordinator <http://www.pulsenetinternational.org/> ?
> EDLB Communications Liaison
>
> Centers for Disease Control and Prevention ?Enteric Diseases Laboratory
> Branch
> P: 404-639-2828 ? F: 404-718-1095 ? E: ypi5 at cdc.gov
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is confidential.
> If you have received this communication in error, please notify us
> immediately and destroy the original message. You may not copy or
> disseminate this communication without the permission of the University.
> Only authorised signatories are competent to enter into agreements on
> behalf of the University and recipients are thus advised that the content
> of this message may not be legally binding on the University and may
> contain the personal views and opinions of the author, which are not
> necessarily the views and opinions of The University of the Witwatersrand,
> Johannesburg. All agreements between the University and outsiders are
> subject to South African Law unless the University agrees in writing to the
> contrary.
>
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From pulsenetafrica at lists.wits.ac.za  Wed Mar 27 09:18:50 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Wed, 27 Mar 2019 07:18:50 +0000
Subject: [PulseNet Africa] PulseNet Quick Tips: Organism Summary Chart for
 WGS
In-Reply-To: <16055309e0c24d21ac626ecc50ca7539@cdc.gov>
References: <16055309e0c24d21ac626ecc50ca7539@cdc.gov>
Message-ID: <mailman.5692.1553671927.10460.pulsenetafrica@lists.wits.ac.za>


FYI.



Dr. Anthony Smith
Principal Medical Scientist
Centre for Enteric Diseases (Bacteriology Division)
National Institute for Communicable Diseases
South Africa
E-mail: anthonys at nicd.ac.za<mailto:anthonys at nicd.ac.za>
Tel: +27-11-5550348 | Cell: +27-83-4612145
Website: www.nicd.ac.za<http://www.nicd.ac.za/>





From: PulseNet (CDC) <pulsenet at cdc.gov>
Sent: 26 March 2019 10:37 PM
To: PulseNet (CDC) <pulsenet at cdc.gov>
Subject: PulseNet Quick Tips: Organism Summary Chart for WGS



[cid:image001.jpg at 01D4E3F2.1E5477F0]




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[cid:image023.jpg at 01D4E3F2.1E5477F0]




The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Service or its management. The information in this e-mail is confidential and is intended solely for the addressee.
Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful.
Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination.
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From pulsenetafrica at lists.wits.ac.za  Wed Mar 27 14:55:33 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Wed, 27 Mar 2019 14:55:33 +0200
Subject: [PulseNet Africa] PulseNet Quick Tips: Organism Summary Chart
 for WGS
In-Reply-To: <mailman.5692.1553671927.10460.pulsenetafrica@lists.wits.ac.za>
References: <16055309e0c24d21ac626ecc50ca7539@cdc.gov>
 <mailman.5692.1553671927.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.5741.1553690946.10460.pulsenetafrica@lists.wits.ac.za>

Thanks Anthony for the info
Daniel Lawadi Banda
Senior Lecturer in Medical Microbiology
Department of Medical Laboratory Sciences
College of Medicine
University of Malawi



Mobile:   +265 99 729 5898 For calling
              +265 88 459 6936 For calling
              +27 622946654 For WhatsApp
Skype:daniel.banda3



On Wed, Mar 27, 2019 at 9:38 AM <pulsenetafrica at lists.wits.ac.za> wrote:

> FYI.
>
>
>
>
>
>
>
> *Dr. Anthony Smith*
>
> Principal Medical Scientist
>
> Centre for Enteric Diseases (Bacteriology Division)
>
> National Institute for Communicable Diseases
>
> South Africa
>
> E-mail: anthonys at nicd.ac.za
>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
>
> Website: www.nicd.ac.za
>
>
>
>
>
>
>
>
>
>
>
> *From:* PulseNet (CDC) <pulsenet at cdc.gov>
> *Sent:* 26 March 2019 10:37 PM
> *To:* PulseNet (CDC) <pulsenet at cdc.gov>
> *Subject:* PulseNet Quick Tips: Organism Summary Chart for WGS
>
>
>
>
>
>
> The views expressed in this email are, unless otherwise stated, those of
> the author and not those of the National Health Laboratory Service or its
> management. The information in this e-mail is confidential and is intended
> solely for the addressee. Access to this e-mail by anyone else is
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From pulsenetafrica at lists.wits.ac.za  Thu Mar 28 13:40:04 2019
From: pulsenetafrica at lists.wits.ac.za (pulsenetafrica at lists.wits.ac.za)
Date: Thu, 28 Mar 2019 11:40:04 +0000 (UTC)
Subject: [PulseNet Africa] PulseNet Quick Tips: Organism Summary Chart
 for WGS
In-Reply-To: <mailman.5741.1553690946.10460.pulsenetafrica@lists.wits.ac.za>
References: <16055309e0c24d21ac626ecc50ca7539@cdc.gov>
 <mailman.5692.1553671927.10460.pulsenetafrica@lists.wits.ac.za>
 <mailman.5741.1553690946.10460.pulsenetafrica@lists.wits.ac.za>
Message-ID: <mailman.5873.1553772870.10460.pulsenetafrica@lists.wits.ac.za>

 Many thanks Anthony.Stella

    On Wednesday, March 27, 2019, 1:55:13 PM GMT+1, <pulsenetafrica at lists.wits.ac.za> wrote:  
 
 Thanks Anthony for the info
Daniel Lawadi BandaSenior Lecturer in Medical MicrobiologyDepartment of Medical Laboratory SciencesCollege of MedicineUniversity of Malawi


Mobile: ? +265 99 729 5898 For calling? ? ? ? ? ? ? +265 88 459 6936 For calling? ? ? ? ? ? ? +27 622946654 For WhatsAppSkype:daniel.banda3? ? ? ? ?

On Wed, Mar 27, 2019 at 9:38 AM <pulsenetafrica at lists.wits.ac.za> wrote:


FYI.

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?

?

Dr. Anthony Smith

Principal Medical Scientist

Centre for Enteric Diseases (Bacteriology Division)

National Institute for Communicable Diseases

South Africa

E-mail:anthonys at nicd.ac.za

Tel: +27-11-5550348 | Cell: +27-83-4612145

Website:www.nicd.ac.za

?

?

?

?

?

From: PulseNet (CDC) <pulsenet at cdc.gov>
Sent: 26 March 2019 10:37 PM
To: PulseNet (CDC) <pulsenet at cdc.gov>
Subject: PulseNet Quick Tips: Organism Summary Chart for WGS

?

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The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Service or its management. The information in this e-mail is confidential and is intended solely for the addressee.Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful.Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination._______________________________________________
Pulsenetafrica mailing list
Pulsenetafrica at lists.wits.ac.za
http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorised signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary.

_______________________________________________
Pulsenetafrica mailing list
Pulsenetafrica at lists.wits.ac.za
http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
This communication is intended for the addressee only. It is confidential. If you have received this communication in error, please notify us immediately and destroy the original message. You may not copy or disseminate this communication without the permission of the University. Only authorised signatories are competent to enter into agreements on behalf of the University and recipients are thus advised that the content of this message may not be legally binding on the University and may contain the personal views and opinions of the author, which are not necessarily the views and opinions of The University of the Witwatersrand, Johannesburg. All agreements between the University and outsiders are subject to South African Law unless the University agrees in writing to the contrary.
  
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