[PulseNet Africa] New PulseNet WGS protocols

[pulsenetafrica at lists.wits.ac.za]

Thanks - well received


On March 15, 2019 09:38:01 am GMT+2, pulsenetafrica at lists.wits.ac.za wrote:

> FYI.
>
> Dr. Anthony Smith
> Principal Medical Scientist
> Centre for Enteric Diseases (Bacteriology Division)
> National Institute for Communicable Diseases
> South Africa
> E-mail: anthonys at nicd.ac.za<mailto:anthonys at nicd.ac.za>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
> Website: www.nicd.ac.za<http://www.nicd.ac.za/>
>
>
> From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
> Sent: 14 March 2019 09:04 PM
> To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen 
> <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) 
> <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. 
> Enrique (WDC) <pereze at paho.org>; Amina AlJardani 
> <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 
> 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin 
> <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle 
> <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; 
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, 
> Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> Subject: New PulseNet WGS protocols
>
> Dear all:
> PulseNet USA has released several new WGS protocols. The original 
> PNL32 protocol has been replaced by individual protocols for the 
> different components of the WGS workflow. A procedure for the use of 
> Illumina’s DNA Flex has also been released. We have received great 
> feedback from the states on the use of the DNA Flex Library Prep kit.
> Attached, please find all the protocols listed below. Feel free to 
> share these with your network participants. In the near future, these 
> protocols will be available on the PulseNet website. Let us know if 
> you have questions about these protocols.
>
> ·         PNL33 - DNA Extraction and Quality
>
> ·         PNL34 – PN Nextera XT Library Prep
>
> ·         PNL35 – DNA Flex Library Prep
>
> ·         PNL38 -  WGS on MiSeq
> Best,
> Jeny
>
> Jeniffer Concepción-Acevedo, Ph.D.
> PulseNet International 
> Coordinator<http://www.pulsenetinternational.org/> │ EDLB 
> Communications Liaison
> Centers for Disease Control and Prevention │Enteric Diseases 
> Laboratory Branch
> P: 404-639-2828 │ F: 404-718-1095 │ E: ypi5 at cdc.gov<mailto:ypi5 at cdc.gov>
>
>
> _______________________________________________
> Pulsenetafrica mailing list
> Pulsenetafrica at lists.wits.ac.za
> http://lists.wits.ac.za/mailman/listinfo/pulsenetafrica
> This communication is intended for the addressee only. It is 
> confidential. If you have received this communication in error, 
> please notify us immediately and destroy the original message. You 
> may not copy or disseminate this communication without the permission 
> of the University. Only authorised signatories are competent to enter 
> into agreements on behalf of the University and recipients are thus 
> advised that the content of this message may not be legally binding 
> on the University and may contain the personal views and opinions of 
> the author, which are not necessarily the views and opinions of The 
> University of the Witwatersrand, Johannesburg. All agreements between 
> the University and outsiders are subject to South African Law unless 
> the University agrees in writing to the contrary.


--
Andrew Tarupiwa
tarupiwaa at nmrl.org.zw