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</o:shapelayout></xml><![endif]--></head><body lang=EN-ZA link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal><span style='font-size:13.0pt;line-height:115%;font-family:"Times New Roman","serif";color:black'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:13.0pt;line-height:115%;font-family:"Times New Roman","serif";color:black'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm'><p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal'><b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span lang=EN-US style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> PFGE National Database (CDC) [mailto:pfge@cdc.gov] <br><b>Sent:</b> 05 January 2016 07:51 PM<br><b>To:</b> PFGE National Database (CDC)<br><b>Subject:</b> PulseNet Quick Tip: PFGE Equipment Maintenance Series, Replacing Electrodes<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
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" v:shapes="Picture_x0020_7"></span><![endif]><img width=261 height=365 id="_x0000_i1025" src="cid:image021.jpg@01D147B4.7D422FB0" alt="cid:image009.jpg@01D14798.0DA8EA70"><o:p></o:p></p></td></tr><tr style='height:216.75pt'><td width=894 valign=top style='width:670.5pt;border:double #4472C4 1.5pt;border-top:none;background:#EDEDED;padding:0cm 5.4pt 0cm 5.4pt;height:216.75pt'><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo2'><![if !supportLists]><span style='font-size:11.0pt;font-family:"Calibri","sans-serif"'><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-family:"Calibri","sans-serif"'>Disconnect the power supply and tubing from the electrophoresis chamber. Turn over the electrophoresis chamber, unscrew the feet, and remove the screws holding the base plate with a Philips screwdriver. Remove the base plate.<o:p></o:p></span></p><p class=MsoNormal><!--[if gte vml 1]><v:shape id="Right_x0020_Arrow_x0020_42" o:spid="_x0000_s1026" type="#_x0000_t75" style='position:absolute;margin-left:242.5pt;margin-top:50.3pt;width:75.75pt;height:36pt;z-index:251659264;visibility:visible;mso-wrap-style:square;mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;mso-position-horizontal-relative:text;mso-position-vertical-relative:text;mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'>
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</v:shape><![endif]--><![if !vml]><span style='mso-ignore:vglayout;position:absolute;z-index:251663360;margin-left:375px;margin-top:87px;width:101px;height:48px'><img width=101 height=48 src="cid:image030.png@01D147B4.7D422FB0" v:shapes="Right_x0020_Arrow_x0020_198"></span><![endif]> <span style='color:#1F497D'> </span> <img width=195 height=249 id="_x0000_i1031" src="cid:image037.jpg@01D147B4.7D422FB0" alt=þÿ> <img width=198 height=254 id="_x0000_i1032" src="cid:image038.jpg@01D147B4.7D422FB0" alt=þÿ><span style='font-size:12.0pt;line-height:115%'><o:p></o:p></span></p></td></tr><tr style='height:195.6pt'><td width=894 valign=top style='width:670.5pt;border:double #4472C4 1.5pt;border-top:none;background:#EDEDED;padding:0cm 5.4pt 0cm 5.4pt;height:195.6pt'><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo2'><![if !supportLists]><span style='font-size:11.0pt;font-family:"Calibri","sans-serif"'><span style='mso-list:Ignore'>5.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-family:"Calibri","sans-serif"'>Replace the washer, hexagonal nut, wire, and second nut. Replace the base plate/feet and reconnect the tubing/power supply.</span><o:p></o:p></p><p class=MsoNormal><!--[if gte vml 1]><v:shape id="Right_x0020_Arrow_x0020_199" o:spid="_x0000_s1029" type="#_x0000_t75" style='position:absolute;margin-left:284.9pt;margin-top:50.45pt;width:75.75pt;height:36pt;z-index:251665408;visibility:visible;mso-wrap-style:square;mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;mso-position-horizontal-relative:text;mso-position-vertical-relative:text;mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;mso-height-relative:page'>
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<o:lock v:ext="edit" aspectratio="f" />
</v:shape><![endif]--><![if !vml]><span style='mso-ignore:vglayout;position:absolute;z-index:251665408;margin-left:380px;margin-top:67px;width:101px;height:48px'><img width=101 height=48 src="cid:image030.png@01D147B4.7D422FB0" v:shapes="Right_x0020_Arrow_x0020_199"></span><![endif]><span style='color:#1F497D'> </span> <img width=212 height=227 id="_x0000_i1033" src="cid:image039.jpg@01D147B4.7D422FB0" alt=þÿ> <img width=268 height=227 id="_x0000_i1034" src="cid:image040.png@01D147B4.7D422FB0" alt="cid:image021.png@01D14798.0DA8EA70"><span style='font-size:12.0pt;line-height:115%'><o:p></o:p></span></p></td></tr><tr style='height:230.35pt'><td width=894 valign=top style='width:670.5pt;border:double #4472C4 1.5pt;border-top:none;background:#EDEDED;padding:0cm 5.4pt 0cm 5.4pt;height:230.35pt'><p class=MsoListParagraphCxSpFirst><span style='font-family:"Calibri","sans-serif"'>Materials and Equipment Needed: <o:p></o:p></span></p><p class=MsoListParagraphCxSpMiddle><!--[if gte vml 1]><v:shape id="object_x0020_3" o:spid="_x0000_s1030" type="#_x0000_t75" style='position:absolute;left:0;text-align:left;margin-left:326.3pt;margin-top:12.35pt;width:139.5pt;height:108.15pt;z-index:251667456;visibility:visible;mso-wrap-style:square;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;mso-position-horizontal-relative:text;mso-position-vertical-relative:text;mso-width-relative:page;mso-height-relative:page'>
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</v:shape><![endif]--><![if !vml]><span style='mso-ignore:vglayout'><table cellpadding=0 cellspacing=0 align=left><tr><td width=435 height=17></td></tr><tr><td></td><td><img width=186 height=144 src="cid:image007.png@01D14867.4949CDE0" v:shapes="object_x0020_3"></td></tr></table></span><![endif]><span style='font-family:"Calibri","sans-serif"'><o:p> </o:p></span></p><br style='mso-ignore:vglayout' clear=ALL><p class=MsoListParagraphCxSpLast><!--[if gte vml 1]><v:shape id="_x0000_s1035" type="#_x0000_t75" alt="-Self-leveling clear silicone
-Philips head screw driver
Hex screwdriver, ¼”
" style='position:absolute;left:0;text-align:left;margin-left:321.7pt;margin-top:103.85pt;width:219.85pt;height:64.8pt;z-index:251677696;visibility:visible;mso-width-percent:0;mso-height-percent:0;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;mso-position-horizontal:absolute;mso-position-horizontal-relative:text;mso-position-vertical:absolute;mso-position-vertical-relative:text;mso-width-percent:0;mso-height-percent:0;mso-width-relative:margin;mso-height-relative:margin'>
<v:imagedata src="cid:image008.emz@01D14867.4949CDE0" o:title="" />
</v:shape><![endif]--><![if !vml]><span style='mso-ignore:vglayout;position:absolute;z-index:251677696;left:0px;margin-left:429px;margin-top:139px;width:293px;height:86px'><img width=293 height=86 src="cid:image010.png@01D14867.4949CDE0" alt="-Self-leveling clear silicone
-Philips head screw driver
Hex screwdriver, ¼”
" v:shapes="_x0000_s1035"></span><![endif]><img width=254 height=176 id="_x0000_i1035" src="cid:image045.jpg@01D147B4.7D422FB0" alt="cid:image024.jpg@01D14798.0DA8EA70"><span style='font-family:"Calibri","sans-serif"'><o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal'><span style='font-size:12.0pt'> <span style='color:#1F497D'> </span> <span style='color:#1F497D'>-</span> Replacement electrodes <o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal'><span style='font-size:12.0pt'> <span style='color:#1F497D'> </span> Bio-Rad catalog #1703648 <o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal'><span style='font-size:12.0pt'> <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> Thank you,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'> <span style='color:#1F497D'> </span>PulseNet<o:p></o:p></span></p></td></tr></table><p class=MsoNormal><!--[if gte vml 1]><v:shape id="Picture_x0020_8" o:spid="_x0000_s1033" type="#_x0000_t75" alt="QuickTips_Footer" style='position:absolute;margin-left:6.75pt;margin-top:2.1pt;width:672.45pt;height:50.05pt;z-index:251674624;visibility:visible;mso-wrap-style:square;mso-height-percent:0;mso-wrap-distance-left:2.88pt;mso-wrap-distance-top:2.88pt;mso-wrap-distance-right:2.88pt;mso-wrap-distance-bottom:2.88pt;mso-position-horizontal-relative:text;mso-position-vertical-relative:text;mso-height-percent:0;mso-width-relative:page;mso-height-relative:page' filled="t" fillcolor="#17375e">
<v:imagedata src="cid:image046.jpg@01D147B4.7D422FB0" o:title="QuickTips_Footer" />
</v:shape><![endif]--><![if !vml]><span style='mso-ignore:vglayout'><table cellpadding=0 cellspacing=0 align=left><tr><td width=9 height=3></td></tr><tr><td></td><td><img width=897 height=67 src="cid:image012.jpg@01D14867.4949CDE0" alt="QuickTips_Footer" v:shapes="Picture_x0020_8"></td></tr></table></span><![endif]><span lang=EN-US><o:p> </o:p></span></p></div>The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Service or its management. The information in this e-mail is confidential and is intended solely for the addressee.
Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful.
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