[PulseNet Africa] Shiga toxigenic E coli

Tue Oct 28 09:20:16 SAST 2014

Sunita,

Try the WHO Collaborating Centre for Reference and Research on Escherichia and Klebsiella, Statens Serum Institut, Denmark: http://www.ssi.dk/English/HealthdataandICT/National%20Reference%20Laboratories/Bacteria/WHO%20Collaborating%20Centre%20for%20Reference%20and%20Research%20on%20Escherichia%20and%20Klebsiella.aspx

Regards,

Anthony

From: pulsenetafrica at lists.wits.ac.za [mailto:pulsenetafrica at lists.wits.ac.za] 
Sent: 28 October 2014 09:06 AM
To: pulsenetafrica at lists.wits.ac.za
Subject: [PulseNet Africa] Shiga toxigenic E coli

Dear all,

We are currently working on isolation and characterisation of Shiga toxigenic E.coli, focusing on the big six and O157:H7.  We've been trying to obtain STEC DNA to use as positive control for PCR assays with the genes stx1,stx2,eaeA and hlyA and other serogroup-specific genes, with not much success.

Grateful if anyone could help us. 

Thank you

Sunita

Mrs Sunita J Santchurn, PhD

Senior Lecturer (Food Science & Technology)

Department of Agricultural & Food Science
Faculty of Agriculture
University of Mauritius
Réduit
Republic of Mauritius

Tel: +230 403 7656 
Fax: +230 465 5743
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