[PulseNet Africa] New PulseNet WGS protocols

[pulsenetafrica at lists.wits.ac.za]

Dear Sir
We are very grateful
Yours faithfully
Najjuka

On Fri, Mar 15, 2019 at 10:37 AM <pulsenetafrica at lists.wits.ac.za> wrote:

> FYI.
>
>
>
> *Dr. Anthony Smith*
>
> Principal Medical Scientist
>
> Centre for Enteric Diseases (Bacteriology Division)
>
> National Institute for Communicable Diseases
>
> South Africa
>
> E-mail: anthonys at nicd.ac.za
>
> Tel: +27-11-5550348 | Cell: +27-83-4612145
>
> Website: www.nicd.ac.za
>
>
>
>
>
> *From:* Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
>
> *Sent:* 14 March 2019 09:04 PM
> *To:* Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <
> ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>;
> Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <
> pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <
> rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>;
> Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <
> Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
> *Cc:* Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>;
> Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
> (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
> *Subject:* New PulseNet WGS protocols
>
>
>
> Dear all:
>
> PulseNet USA has released several new WGS protocols. The original PNL32
> protocol has been replaced by individual protocols for the different
> components of the WGS workflow. A procedure for the use of Illumina’s DNA
> Flex has also been released. We have received great feedback from the
> states on the use of the DNA Flex Library Prep kit.
>
> Attached, please find all the protocols listed below. Feel free to share
> these with your network participants. In the near future, these protocols
> will be available on the PulseNet website. Let us know if you have
> questions about these protocols.
>
> ·         PNL33 - DNA Extraction and Quality
>
> ·         PNL34 – PN Nextera XT Library Prep
>
> ·         PNL35 – DNA Flex Library Prep
>
> ·         PNL38 -  WGS on MiSeq
>
> Best,
>
> Jeny
>
>
>
> *Jeniffer Concepci**ó**n-Acevedo, Ph.D.*
>
> PulseNet International Coordinator <http://www.pulsenetinternational.org/> │
> EDLB Communications Liaison
>
> Centers for Disease Control and Prevention │Enteric Diseases Laboratory
> Branch
> P: 404-639-2828 │ F: 404-718-1095 │ E: ypi5 at cdc.gov
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