[PulseNet Africa] New PulseNet WGS protocols

Fri Mar 15 10:15:38 SAST 2019

 Dear Dr Anthony
Very great information and thank you
Best RegardsFirehiwot.

    On Friday, March 15, 2019, 10:38:00 AM GMT+3, pulsenetafrica at lists.wits.ac.za <pulsenetafrica at lists.wits.ac.za> wrote:  

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FYI.

Dr. Anthony Smith

Principal Medical Scientist

Centre for Enteric Diseases (Bacteriology Division)

National Institute for Communicable Diseases

South Africa

E-mail: anthonys at nicd.ac.za

Tel: +27-11-5550348 | Cell: +27-83-4612145

Website: www.nicd.ac.za

From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
Sent: 14 March 2019 09:04 PM
To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
Subject: New PulseNet WGS protocols

Dear all:

PulseNet USA has released several new WGS protocols. The original PNL32 protocol has been replaced by individual protocols for the different components of the WGS workflow. A procedure for the use of Illumina’s DNA Flex has also been released. We have received great feedback from the states on the use of the DNA Flex Library Prep kit.

Attached, please find all the protocols listed below. Feel free to share these with your network participants. In the near future, these protocols will be available on the PulseNet website. Let us know if you have questions about these protocols.

·        PNL33 - DNA Extraction and Quality

·        PNL34 – PN Nextera XT Library Prep

·        PNL35 – DNA Flex Library Prep

·        PNL38 -  WGS on MiSeq

Best,

Jeny

Jeniffer Concepción-Acevedo, Ph.D.

PulseNet International Coordinator│ EDLB Communications Liaison 

Centers for Disease Control and Prevention │Enteric Diseases Laboratory Branch
P: 404-639-2828 │ F: 404-718-1095 │ E: ypi5 at cdc.gov
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