[PulseNet Africa] New PulseNet WGS protocols

[pulsenetafrica at lists.wits.ac.za]

FYI.

Dr. Anthony Smith
Principal Medical Scientist
Centre for Enteric Diseases (Bacteriology Division)
National Institute for Communicable Diseases
South Africa
E-mail: anthonys at nicd.ac.za<mailto:anthonys at nicd.ac.za>
Tel: +27-11-5550348 | Cell: +27-83-4612145
Website: www.nicd.ac.za<http://www.nicd.ac.za/>


From: Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED) <ypi5 at cdc.gov>
Sent: 14 March 2019 09:04 PM
To: Nadon, Celine (PHAC/ASPC) <celine.nadon at canada.ca>; Isabel Chinen <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof. (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC) <pereze at paho.org>; Amina AlJardani <aksaljardani at gmail.com>; Rajesh Kumar <rajeshverma2005 at gmail.com>; 'Josefina Campos' <jocampos05 at gmail.com>; Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle <Ivo.Van.Walle at ecdc.europa.eu>; Johanna.Takkinen at ecdc.europa.eu
Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED) <evy7 at cdc.gov>; Gerner-Smidt, Peter (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
Subject: New PulseNet WGS protocols

Dear all:
PulseNet USA has released several new WGS protocols. The original PNL32 protocol has been replaced by individual protocols for the different components of the WGS workflow. A procedure for the use of Illumina’s DNA Flex has also been released. We have received great feedback from the states on the use of the DNA Flex Library Prep kit.
Attached, please find all the protocols listed below. Feel free to share these with your network participants. In the near future, these protocols will be available on the PulseNet website. Let us know if you have questions about these protocols.

·         PNL33 - DNA Extraction and Quality

·         PNL34 – PN Nextera XT Library Prep

·         PNL35 – DNA Flex Library Prep

·         PNL38 -  WGS on MiSeq
Best,
Jeny

Jeniffer Concepción-Acevedo, Ph.D.
PulseNet International Coordinator<http://www.pulsenetinternational.org/> │ EDLB Communications Liaison
Centers for Disease Control and Prevention │Enteric Diseases Laboratory Branch
P: 404-639-2828 │ F: 404-718-1095 │ E: ypi5 at cdc.gov<mailto:ypi5 at cdc.gov>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.wits.ac.za/pipermail/pulsenetafrica/attachments/20190315/967dd5c2/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: PNL33-DNA Extraction and Quality.pdf
Type: application/pdf
Size: 538835 bytes
Desc: PNL33-DNA Extraction and Quality.pdf
URL: <http://lists.wits.ac.za/pipermail/pulsenetafrica/attachments/20190315/967dd5c2/attachment-0004.pdf>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: PNL38-WGS on MiSeq.pdf
Type: application/pdf
Size: 338035 bytes
Desc: PNL38-WGS on MiSeq.pdf
URL: <http://lists.wits.ac.za/pipermail/pulsenetafrica/attachments/20190315/967dd5c2/attachment-0005.pdf>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: PNL34-PN Nextera XT Library Prep.pdf
Type: application/pdf
Size: 399427 bytes
Desc: PNL34-PN Nextera XT Library Prep.pdf
URL: <http://lists.wits.ac.za/pipermail/pulsenetafrica/attachments/20190315/967dd5c2/attachment-0006.pdf>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: PNL35_DNA_Flex_Protocol_Lib_Prep.pdf
Type: application/pdf
Size: 392185 bytes
Desc: PNL35_DNA_Flex_Protocol_Lib_Prep.pdf
URL: <http://lists.wits.ac.za/pipermail/pulsenetafrica/attachments/20190315/967dd5c2/attachment-0007.pdf>