[PulseNet Africa] New PulseNet WGS protocols

Sat Mar 16 15:34:09 SAST 2019

Dear Anthony,  Thank you for this communication. Stephen Ndibowa 
--------------------------------------------
On Fri, 3/15/19,  <pulsenetafrica at lists.wits.ac.za> wrote:

 Subject: Re: [PulseNet Africa] New PulseNet WGS protocols
 To: "pulsenetafrica at lists.wits.ac.za" <pulsenetafrica at lists.wits.ac.za>
 Date: Friday, March 15, 2019, 6:16 PM

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 Dear
 ANthony,thank you.Best

 Dr Kalpy
 Julien COULIBALY, MD, MSc FS, PhD
 Médecin Microbiologiste / Chargé de Recherche
 Chef de Département Environnement et Santé 
 Institut Pasteur de Côte
 d'Ivoire
 01 BP 490 Abidjan 01 

 Centre Médico-Social
 Université Félix
 Houphouet-Boigny
 BP V 34 Abidjan
 kalpyjuliencoulibaly at pasteur.ci
 0022507915176

                     Le vendredi 15 mars 2019 à 07:38:00
 UTC, pulsenetafrica at lists.wits.ac.za
 <pulsenetafrica at lists.wits.ac.za> a écrit :

 FYI. 

 Dr.
 Anthony Smith

 Principal
 Medical Scientist 

 Centre
 for Enteric Diseases (Bacteriology Division) 

 National
 Institute for Communicable Diseases 

 South
 Africa 

 E-mail:

 anthonys at nicd.ac.za

 Tel:
 +27-11-5550348 | Cell: +27-83-4612145 

 Website:

 www.nicd.ac.za 

 From:
 Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED)
 <ypi5 at cdc.gov>

 Sent: 14 March 2019 09:04 PM

 To: Nadon, Celine (PHAC/ASPC)
 <celine.nadon at canada.ca>; Isabel Chinen
 <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof.
 (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith
 <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC)
 <pereze at paho.org>; Amina AlJardani
 <aksaljardani at gmail.com>;
  Rajesh Kumar <rajeshverma2005 at gmail.com>;
 'Josefina Campos' <jocampos05 at gmail.com>;
 Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle
 <Ivo.Van.Walle at ecdc.europa.eu>;
 Johanna.Takkinen at ecdc.europa.eu

 Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED)
 <evy7 at cdc.gov>; Gerner-Smidt, Peter
 (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
 (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>

 Subject: New PulseNet WGS protocols 

 Dear all: 
 PulseNet USA has released several new WGS
 protocols. The original PNL32 protocol has been replaced by
 individual protocols for the different components of the
  WGS workflow. A procedure for the use of Illumina’s DNA
 Flex has also been released. We have received great feedback
 from the states on the use of the DNA Flex Library Prep kit.

 Attached, please find all the protocols listed
 below. Feel free to share these with your network
 participants. In the near future, these protocols will be
 available
  on the PulseNet website. Let us know if you have questions
 about these protocols.

 ·        
 PNL33 - DNA Extraction and Quality 

 ·        
 PNL34 – PN Nextera XT Library Prep

 ·        
 PNL35 – DNA Flex Library Prep 

 ·        
 PNL38 -  WGS on MiSeq 
 Best, 
 Jeny 

 Jeniffer Concepción-Acevedo, Ph.D. 

 PulseNet International Coordinator
 │ EDLB
 Communications Liaison 

 Centers for
 Disease Control and Prevention │Enteric Diseases
 Laboratory Branch

 P: 404-639-2828 │ F: 404-718-1095 │ E: ypi5 at cdc.gov 

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