[PulseNet Africa] New PulseNet WGS protocols
pulsenetafrica at lists.wits.ac.za
pulsenetafrica at lists.wits.ac.za
Mon Mar 18 10:39:50 SAST 2019
Thank Dr Anthony for the information
Daniel Lawadi Banda
Senior Lecturer in Medical Microbiology
Department of Medical Laboratory Sciences
College of Medicine
University of Malawi
Mobile: +265 99 729 5898 For calling
+265 88 459 6936 For calling
+27 622946654 For WhatsApp
Skype:daniel.banda3
On Sat, Mar 16, 2019 at 3:36 PM <pulsenetafrica at lists.wits.ac.za> wrote:
> Dear Anthony, Thank you for this communication. Stephen Ndibowa
> --------------------------------------------
> On Fri, 3/15/19, <pulsenetafrica at lists.wits.ac.za> wrote:
>
> Subject: Re: [PulseNet Africa] New PulseNet WGS protocols
> To: "pulsenetafrica at lists.wits.ac.za" <pulsenetafrica at lists.wits.ac.za>
> Date: Friday, March 15, 2019, 6:16 PM
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> Dear
> ANthony,thank you.Best
>
> Dr Kalpy
> Julien COULIBALY, MD, MSc FS, PhD
> Médecin Microbiologiste / Chargé de Recherche
> Chef de Département Environnement et Santé
> Institut Pasteur de Côte
> d'Ivoire
> 01 BP 490 Abidjan 01
>
> Centre Médico-Social
> Université Félix
> Houphouet-Boigny
> BP V 34 Abidjan
> kalpyjuliencoulibaly at pasteur.ci
> 0022507915176
>
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> Le vendredi 15 mars 2019 à 07:38:00
> UTC, pulsenetafrica at lists.wits.ac.za
> <pulsenetafrica at lists.wits.ac.za> a écrit :
>
>
>
>
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> FYI.
>
>
> Dr.
> Anthony Smith
>
>
> Principal
> Medical Scientist
>
> Centre
> for Enteric Diseases (Bacteriology Division)
>
> National
> Institute for Communicable Diseases
>
> South
> Africa
>
> E-mail:
>
> anthonys at nicd.ac.za
>
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> Tel:
> +27-11-5550348 | Cell: +27-83-4612145
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> Website:
>
> www.nicd.ac.za
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>
>
>
> From:
> Concepcion Acevedo, Jeniffer (CDC/DDID/NCEZID/DFWED)
> <ypi5 at cdc.gov>
>
>
> Sent: 14 March 2019 09:04 PM
>
> To: Nadon, Celine (PHAC/ASPC)
> <celine.nadon at canada.ca>; Isabel Chinen
> <ichinen at anlis.gov.ar>; Joseph Kai Man Kam, Prof.
> (CEID) <kmkam at cuhk.edu.hk>; Anthony Smith
> <anthonys at nicd.ac.za>; Perez, Dr. Enrique (WDC)
> <pereze at paho.org>; Amina AlJardani
> <aksaljardani at gmail.com>;
> Rajesh Kumar <rajeshverma2005 at gmail.com>;
> 'Josefina Campos' <jocampos05 at gmail.com>;
> Brent Gilpin <Brent.Gilpin at esr.cri.nz>; Ivo Van Walle
> <Ivo.Van.Walle at ecdc.europa.eu>;
> Johanna.Takkinen at ecdc.europa.eu
>
> Cc: Freeman, Molly (CDC/DDID/NCEZID/DFWED)
> <evy7 at cdc.gov>; Gerner-Smidt, Peter
> (CDC/DDID/NCEZID/DFWED) <plg5 at cdc.gov>; Trees, Eija
> (CDC/DDID/NCEZID/DFWED) <eih9 at cdc.gov>
>
> Subject: New PulseNet WGS protocols
>
>
>
> Dear all:
> PulseNet USA has released several new WGS
> protocols. The original PNL32 protocol has been replaced by
> individual protocols for the different components of the
> WGS workflow. A procedure for the use of Illumina’s DNA
> Flex has also been released. We have received great feedback
> from the states on the use of the DNA Flex Library Prep kit.
>
> Attached, please find all the protocols listed
> below. Feel free to share these with your network
> participants. In the near future, these protocols will be
> available
> on the PulseNet website. Let us know if you have questions
> about these protocols.
>
>
> ·
> PNL33 - DNA Extraction and Quality
>
> ·
> PNL34 – PN Nextera XT Library Prep
>
>
> ·
> PNL35 – DNA Flex Library Prep
>
> ·
> PNL38 - WGS on MiSeq
> Best,
> Jeny
>
>
>
> Jeniffer Concepción-Acevedo, Ph.D.
>
> PulseNet International Coordinator
> │ EDLB
> Communications Liaison
>
>
> Centers for
> Disease Control and Prevention │Enteric Diseases
> Laboratory Branch
>
> P: 404-639-2828 │ F: 404-718-1095 │ E: ypi5 at cdc.gov
>
>
>
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